3.4.1 Explain DNA replication in terms of unwinding the double helix and separation of the strands by helicase, followed by formation of the new complementary strands by DNA polymerase.
DNA replication is semi-conservative as both of the DNA molecules produced are formed from an old strand and a new one. The first stage of DNA replication involves the unwinding of the double strand of DNA (DNA double helix) and separating them by breaking the hydrogen bonds between the bases. This is done by the enzyme helicase. Each separated strand now is a template for the new strands. There are many free nucleotides around the replication fork which then bond to the template strands. The free nucleotides form hydrogen bonds with their complimentary base pairs on the template strand. Adenine will pair up with thymine and guanine will pair up with cytosine. DNA polymerase is the enzyme responsible for this. The new DNA strands then rewind to form a double helix. The replication process has produced a new DNA molecule which is identical to the initial one.
3.4.2 Explain the significance of complementary base pairing in the conservation of the base sequence of DNA.
Complementary base pairing is very important in the conservation of the base sequence of DNA. This is because adenine always pairs up with thymine and guanine always pairs up with cytosine. As DNA replication is semi-conservative (one old strand an d one new strand make up the new DNA molecules), this complementary base pairing allows the two DNA molecules to be identical to each other as they have the same base sequence. The new strands formed are complementary to their template strands but also identical to the other template. Therefore, complementary base pairing has a big role in the conservation of the base sequence of DNA.
3.4.3 State that DNA replication is semi- conservative.
DNA replication is semi-conservative.